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E-raamat: VEGF Signaling: Methods and Protocols

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  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2475
  • Ilmumisaeg: 22-Apr-2022
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071622179
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VEGF Signaling: Methods and ProtocolsSuurem pilt
  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2475
  • Ilmumisaeg: 22-Apr-2022
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071622179
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This volume provides an updated collection of protocols for manipulating and studying VEGF signaling pathways in vitro and in vivo and aims to present a range of both firmly established and newly emerging technologies. Covering multiple model species, from mouse to zebrafish to human, the book explores the role of VEGF and VEGFR isoforms in exosomes, cultured cells, or in tissues, as well as robust cell assays for the investigation of basic angiogenic mechanisms and VEGF signaling in more complex cellular systems, amongst other subjects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, VEGF Signaling: Methods and Protocols, Second Edition provides a useful tool for researchers in the vascular biology community and beyond in understanding the basic biology of VEGF signaling and in translating this research into the clinic.
Opposing Roles of Vascular Endothelial Growth Factor C in Metastatic
Dissemination and Resistance to Radio/Chemotherapy: Discussion of Mechanisms
and Therapeutic Strategies.- Identification of VEGF Isoforms in Mouse, Rat,
and Zebrafish Using RT-qPCR.- Multi-Parameter Fluorescence-Activated Cell
Sorting of Human Lymphatic Endothelial Cells.- Absolute Quantification of
Plasma Membrane Receptors via Quantitative Flow Cytometry.- Measurement of
VEGF Content in Exosomes and Subsequent Tumor Tubulogenesis and In Vivo
Angiogenesis Functional Assays.- SH2-Domain Protein Isolation Using Synthetic
Phosphorylated Peptides to Study VEGFR2 Signaling.- Monitoring
VEGF-Stimulated Calcium Ion Flux in Endothelial Cells.-
Co-Immunoprecipitation Assays.- Using Immortalized Endothelial Cells to Study
the Roles of Adhesion Molecules in VEGF-Induced Signaling.- RNAscope for
VEGF-A Detection in Human Tumor Bioptic Specimens.- Global MicroRNA Profiling
of Vascular Endothelial Cells.- Endothelial Cell Tube Formation Assay: An In
Vitro Model for Angiogenesis.- Use of a Thin Layer Assay for Assessing the
Angiogenic Potential of Endothelial Cells In Vitro.- VEGF-A165 -Induced
Endothelial Cells Chemotactic Migration and Invasion Assays.- Measuring
Mitochondrial Calcium Fluxes in Cardiomyocyte upon Mechanical Stretch-Induced
Hypertrophy.- Simultaneous Measurement of Endothelial Cell Proliferation and
Cell Cycle Stage Using Flow Cytometry.- Ex Vivo Mouse Aortic Ring
Angiogenesis Assay.- Retinal Microvasculature-on-a-Chip for Modeling
VEGF-Induced Permeability.- Preventing VEGF-Mediated Vascular Permeability by
Experimentally Potentiating BBB Characteristics in Endothelial Cells.- The
Embryonic Mouse Hindbrain and Postnatal Retina as In Vivo Models to Study
Angiogenesis.- Evaluating VEGF-Induced Vascular Leakage Using the Miles
Assay.- Modulation of VEGFA Signaling during Heart Regeneration in
Zebrafish.- Three-Dimensional Visualization of Blood and Lymphatic Vessels in
the Adult Zebrafish Heart by Chemical Clearing.- Fluorescence-Activated Cell
Sorting and Quantitative Real-Time PCR to Reveal VEGF Expressing Macrophage
Populations in the Zebrafish Larvae.- Assessing Molecular Regulation of
Vascular Permeability Using a VEGF-Inducible Zebrafish Model.
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