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E-raamat: Zebrafish: Disease Models and Chemical Screens

Volume editor H. William Detrich III (Professor of Biochemistry and Marine Biology at Northeastern University)

Formaat: EPUB+DRM
Sari: Methods in Cell Biology
Ilmumisaeg: 09-Sep-2011
Kirjastus: Academic Press Inc
Keel: eng
ISBN-13: 9780123813213

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Formaat: EPUB+DRM
Sari: Methods in Cell Biology
Ilmumisaeg: 09-Sep-2011
Kirjastus: Academic Press Inc
Keel: eng
ISBN-13: 9780123813213

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This volume of Methods in Cell Biology is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish.

* Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
* This volume concentrates on Disease Models and Chemical Screens

Muu info

This volume of Methods in Cell Biology is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004

Contributors

Preface

xxi

Part I Disease Models

1 Zebrafish Models of Germ Cell Tumor

Joanie C. Neumann

Kate Lillard

Vanessa Damoulis

James F. Amatruda

I Overview

(1)

II Germline Development

(5)

III Germ Cell Tumors

(4)

IV Methods for Studying Zebrafish Germ Cells

(13)

Reference

(8)

2 Dissecting Mechanisms of Myelinated Axon Formation Using Zebrafish

Tim Czopka

David A. Lyons

I Introduction

(8)

II Methods and Materials

(18)

III Summary

(12)

Acknowledgments

(1)

References

(12)

3 In vivo Analysis of White Adipose Tissue in Zebrafish

James E.N. Minchin

John F. Rawls

I Introduction

(4)

II Rationale

(2)

III Materials

(4)

IV Methods

(9)

V Summary

(5)

Acknowledgments

(1)

References

(5)

4 Study of Host-Microbe Interactions in Zebrafish

Kathryn Milligan-Myhre

Jeremy R. Charette

Ryan T. Phennicie

W. Zac Stephens

John F. Rawls

Karen Guillemin

Carol H. Kim

I Introduction

(7)

II Laboratory Protocols

(23)

References

111

(7)

5 Hematopoietic Stem Cell Development: Using the Zebrafish to Identify the Signaling Networks and Physical Forces Regulating Hematopoiesis

Wolfram Goessling

Trista E. North

I Developmental Hematopoiesis

118

(1)

II Review of the Literature

119

(3)

III Use of Zebrafish to Investigate HSC Biology

122

(7)

IV Zebrafish Tools and Protocols

129

(8)

References

132

(5)

6 Zebrafish as a Model for Hemorrhagic Stroke

Matthew G. Butler

Aniket V. Gore

Brant M. Weinstein

I Introduction and Basic Concepts

137

(5)

II Maintaining the Barrier Function of Blood Vessels

142

(1)

III Vascular Integrity and Stroke

143

(3)

IV Zebrafish as a Model for Studying Hemorrhage and Stroke

146

(2)

V Studying Vascular Integrity in the Zebrafish

148

(7)

VI Concluding Remarks

155

(9)

References

156

(8)

7 A Zebrafish Model for VHL and Hypoxia Signaling

Ellen van Rooijen

Kirankumar Santhakumar

Ive Logister

Emile Voest

Stefan Schulte-Merker

Rachel Giles

Fredericus van Eeden

I VHL Disease

164

(1)

II Chuvash Polycythemia

165

(1)

III VHL Regulates HIF Signaling

165

(2)

IV HIF-Independent Functions of pVHL

167

(2)

V VHL Animal Models

169

(4)

VI Zebrafish as a New Model for VHL

173

(3)

VII Loss of Vhl Leads to a Systemic Hypoxic Response in Zebrafish

176

(1)

VIII vhl Mutants Develop Chuvash Polycythemia and Blood Cell Maturation Defects

176

(2)

IX Zebrafish vhl Mutants Develop Angiogenesis Defects

178

(1)

X Zebrafish vhl Mutants Develop Pronephros Abnormalities

179

(1)

XI Translation from Fish to Humans

180

(1)

XII Concluding Remarks

181

(11)

Acknowledgments

182

(1)

References

182

(10)

8 Basement Membrane Diseases in Zebrafish

Natalia Martins Feitosa

Rebecca Richardson

Wilhelm Bloch

Matthias Hammerschmidt

I Introduction

192

(2)

II Basement Membrane-Related Zebrafish Pathologies

194

(10)

III Methodology for Zebrafish Studies of Basement Membrane Composition and Function

204

(12)

IV Summary

216

(8)

Acknowledgments

216

(1)

References

216

(8)

9 Zebrafish Provides a Novel Model for Lymphatic Vascular Research

Terhi Karpanen

Stefan Schulte-Merker

I Introduction

224

(1)

II Embryonic Lymphangiogenesis in Zebrafish

225

(5)

III Comparison Between Teleosts and Other Vertebrates

230

(1)

IV The Added Value of Zebrafish

231

(2)

V The Secondary Vessel System in Teleosts

233

(2)

VI Concluding Remarks

235

(1)

VII Methods

235

(6)

Acknowledgments

235

(1)

References

236

(5)

10 Not All Bones are Created Equal - Using Zebrafish and Other Teleost Species in Osteogenesis Research

Alexander Apschner

Stefan Schulte-Merker

P. Eckhard Witten

I Case Studies - Using Zebrafish for Addressing Biomedical Questions

241

(2)

II The Evolution of Skeletal Tissues

243

(1)

III Cartilage and Bone in Teleost Fish

244

(2)

IV Intermediate Skeletal Tissues

246

(1)

V Osteocyte-Containing Bone and Acellular Bone

246

(1)

VI Development of Teleost Vertebral Bodies, A Derived Process

247

(1)

VII Remodeling of the Teleost Skeleton

248

(2)

VIII Conclusions

250

(8)

Acknowledgments

250

(1)

References

250

(8)

11 Zebrafish Assays of Ciliopathies

Norann A. Zaghloul

Nicholas Katsanis

I Introduction

258

(2)

II Methods

260

(7)

III Zebrafish Ciliary Mutant Lines

267

(2)

IV General Considerations/Future Development

269

(5)

Acknowledgment

270

(1)

References

270

(4)

12 Infectious Disease Modeling and Innate Immune Function in Zebrafish Embryos

Chao Cui

Erica L. Benard

Zakia Kanwal

Oliver W. Stockhammer

Michiel van der Vaart

Anna Zakrzewska

Herman P. Spaink

Annemarie H. Meijer

I Introduction

274

(1)

II Observation and Isolation of Innate Immune Cells

275

(11)

III Bacterial Infection Methods

286

(7)

IV Analysis of the Innate Immune Response

293

(8)

V Conclusions

301

(9)

Acknowledgments

302

(1)

References

302

(8)

13 Zebrafish as a Model for the Study of Human Cancer

Julia Etchin

John P. Kanki

A. Thomas Look

I Introduction: Zebrafish as a Cancer Model

310

(3)

II The Genetically Tractable Zebrafish

313

(2)

III Transgenic Models of Oncogenesis

315

(6)

IV Modeling the Loss of Tumor Suppression

321

(4)

V Modeling Tumor Cell Intravasation and Metastasis

325

(2)

VI Tumor-Initiating Cells

327

(2)

VII In vivo Small-Molecule Screens and Drug Discovery

329

(3)

VIII Conclusions

332

(8)

References

332

(8)

14 Generating and Analyzing Fish Models of Melanoma

E. Elizabeth Patton

Marie E. Mathers

Manfred Schartl

I Introduction

340

(5)

II Transgenic Melanoma Lines in Zebrafish and Medaka

345

(5)

III Ultra-violet Irradiation Treatments

350

(1)

IV Basic Melanoma Pathology

351

(3)

V Histology

354

(2)

VI Molecular Analysis

356

(4)

VII RNA and Protein Isolation From Adult Fish Tumors

360

(2)

VIII Conclusions

362

(6)

Acknowledgments

362

(1)

References

363

(5)

15 Screening Pancreatic Oncogenes in Zebrafish Using the Gal4/UAS System

Shu Liu

Steven D. Leach

I Introduction

368

(2)

II Transgenic Zebrafish with Gal4/UAS-Mediated eGFP-KRASG12V Expression in the Exocrine Pancreas

370

(1)

III Identification and Characterization of Pancreatic Tumors

371

(8)

IV Conclusions

379

(5)

Acknowledgments

380

(1)

References

380

(4)

16 Zebrafish Models of Rhabdomyosarcoma

Eleanor Y. Chen

David M. Langenau

I Introduction

384

(3)

II Rationale

387

(1)

III Material and Methods

387

(12)

IV Discussion

399

(5)

Acknowledgments

399

(1)

References

399

(5)

17 Transplantation in Zebrafish

Pulin Li

Richard M. White

Leonard I. Zon

I Introduction

404

(1)

II Rationale

404

(3)

III Methods

407

(6)

IV Discussion

413

(7)

Acknowledgment

415

(1)

References

415

(5)

18 Disease Modeling by Gene Targeting Using MicroRNAs

C.C. Lan

I.U.S. Leong

D. Lai

D.R. Love

I Introduction

420

(2)

II Mechanisms of MicroRNA Silencing

422

(1)

III Dicer and MicroRNAs in Zebrafish Development

423

(1)

IV Development of Vector-Based RNA Interference

423

(3)

V RNA Interference Work in Zebrafish

426

(1)

VI Use of siRNAs in the Zebrafish

427

(2)

VII Materials and Methods

429

(2)

VIII Results

431

(1)

IX Future Directions

432

(6)

References

433

(5)

19 Fluorescent Imaging of Cancer in Zebrafish

Myron S. Ignatius

David M. Langenau

I Introduction

438

(1)

II Fluorescent Proteins and Transgenic Models of Cancer

439

(5)

III Macroscopic Observation of Tumor Growth

444

(2)

IV Microscopic Observation in Tumorigenesis

446

(3)

V Confirming Transformation of Fluorescent-Labeled Tumor Cells by Cell Transplantation into Irradiated Recipient Animals

449

(2)

VI Identifying Tumor-Propagating Cell Subpopulations by Fluorescent Protein Expression and Cell Transplantation into Irradiated Recipient Animals

451

(1)

VII Use of Syngeneic Zebrafish for Cell Transplantation of Fluorescent-Labeled Tumors and Drug Discovery

452

(1)

VIII Cell Transplantation into Syngeneic Zebrafish to Accurately Assess Self-Renewal in Fluorescent-Labeled Cancer

453

(1)

IX Use of Syngeneic Zebrafish for Cell Transplantation: Single Cell Transplants

454

(1)

X Xenograft Transplantation of Fluorescently Labeled Cells into Zebrafish

455

(1)

XI Conclusions

456

(6)

Acknowledgments

457

(1)

References

457

(5)

20 The Role of Fanconi Anemia/BRCA Genes in Zebrafish Sex Determination

Adriana Rodriguez-Mari

John H. Postlethwait

I Introduction

462

(3)

II Results and Discussion

465

(16)

III Summary

481

(13)

Acknowledgments

481

(1)

Acknowledgments

481

(13)

Part II Chemical Screens

21 Chemical Screening in Zebrafish for Novel Biological and Therapeutic Discovery

Justin L. Tan

Leonard I. Zon

I Introduction

494

(1)

II Rationale

494

(2)

III Materials and Methods

496

(13)

IV Discussion/Caveats

509

(4)

V Summary

513

(5)

Acknowledgments

513

(1)

References

514

(4)

22 Using the Zebrafish Photomotor Response for Psychotropic Drug Screening

David Kokel

Randall T. Peterson

I Introduction

518

(1)

II The PMR Behavior

518

(1)

III Methods

519

(4)

IV Discussion

523

(3)

Acknowledgments

523

(1)

References

523

(3)

23 Designing Zebrafish Chemical Screens

Randall T. Peterson

Mark C. Fishman

I Rationale for Conducting Small Molecule Screens With Zebrafish

526

(1)

II Selection of Small Molecule Libraries

526

(3)

III Assay Design: Major Screen Types

529

(4)

IV Screening Methods

533

(2)

V Mechanism of Action Studies

535

(3)

VI Conclusions

538

(5)

References

538

(5)

Index

543

(16)

Volumes in Series

559

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

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