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E-raamat: Cyclic Nucleotide Signaling

Edited by (University of Texas Health Science Center, Houston, USA)
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Showcasing the recent progresses of the field, Cyclic Nucleotide Signalingcovers the major tools and methodologies used in various areas of research. The majority of the chapters are protocol oriented, with the goal of providing clear directions for laboratory use. Students and investigators new to the field will find this book particularly informative, as will scientists already actively researching nucleotide signaling.
Series Preface vii
Preface ix
Editor xi
Contributors xiii
Chapter 1 Discovery of Small Molecule EPAC Specific Modulators by High-Throughput Screening
1(18)
Xiaodong Cheng
Tamara Tsalkova
Fang Mei
Chapter 2 Cyclic Nucleotide Analogs as Pharmacological Tools for Studying Signaling Pathways
19(16)
Elke Butt
Chapter 3 High-Throughput FRET Assays for Fast Time-Dependent Detection of Cyclic AMP in Pancreatic β Cells
35(26)
George G. Holz
Colin A. Leech
Michael W. Roe
Oleg G. Chepurny
Chapter 4 Assessing Cyclic Nucleotide Recognition in Cells: Opportunities and Pitfalls for Selective Receptor Activation
61(20)
Rune Kleppe
Lise Madsen
Lars Herfindal
Jan Haavik
Frode Selheim
Stein Ove Døskeland
Chapter 5 Monitoring Cyclic Nucleotides Using Genetically Encoded Fluorescent Reporters
81(18)
Kirill Gorshkov
Jin Zhang
Chapter 6 Structural Characterization of Epac by X-Ray Crystallography
99(14)
Holger Rehmann
Chapter 7 Sensory Neuron cAMP Signaling in Chronic Pain
113(22)
Niels Eijkelkamp
Pooja Singhmar
Cobi J. Heijnen
Annemieke Kavelaars
Chapter 8 Monitoring Real-Time Cyclic Nucleotide Dynamics in Subcellular Microdomains
135(12)
Zeynep Bastug
Viacheslav O. Nikolaev
Chapter 9 Identifying Complexes of Adenylyl Cyclase with A-Kinase Anchoring Proteins
147(18)
Yong Li
Carmen W. Dessauer
Chapter 10 Assessing Cyclic Nucleotide Binding Domain Allostery and Dynamics by NMR Spectroscopy
165(26)
Bryan VanSchouwen
Madoka Akimoto
Stephen Boulton
Kody Moleschi
Rajanish Giri
Giuseppe Melacini
Chapter 11 A Protocol for Expression and Purification of Cyclic Nucleotide--Free Protein in Escherichia coli
191(12)
Jeong Joo Kim
Gilbert Y. Huang
Robert Rieger
Antonius Roller
Dar-Chone Chow
Choel Kim
Chapter 12 Cyclic Nucleotide Analogues as Chemical Tools for Interaction Analysis
203(22)
Robin Lorenz
Claudia Hahnefeld
Stefan Moller
Daniela Bertinetti
Friedrich W. Herberg
Chapter 13 Dissecting the Physiological Functions of PKA Using Genetically Modified Mice
225(30)
Brian W. Jones
Jennifer Deem
Linghai Yang
G. Stanley McKnight
Index 255
Cheng, Xiaodong
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