| Preface |
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| Contributors |
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xi | |
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1 | (23) |
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1 | (2) |
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Methods for interrogating SNPs |
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3 | (4) |
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Commercial platforms for SNP genotyping |
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7 | (4) |
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Practical recommendations |
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11 | (3) |
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14 | (1) |
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15 | (1) |
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Copy number variation analysis |
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16 | (1) |
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Second generation sequencing technologies |
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17 | (1) |
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18 | (1) |
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19 | (5) |
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2 DNA Chip Analysis in Genome Discovery |
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24 | (19) |
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24 | (1) |
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25 | (3) |
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Cross-species hybridization |
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28 | (3) |
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Comparative genomic hybridization and microarray-based genotyping |
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31 | (3) |
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Barcodes, DNA microarrays and organism identification |
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34 | (2) |
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36 | (1) |
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37 | (6) |
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3 qPCR, Theory, Reliability and Use in Molecular Analysis |
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43 | (13) |
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44 | (1) |
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45 | (1) |
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46 | (1) |
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47 | (4) |
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Transparency of published data |
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51 | (1) |
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52 | (1) |
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52 | (1) |
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52 | (4) |
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4 DNA Analysis in Droplet-Based Microfluidic Devices |
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56 | (25) |
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56 | (2) |
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Continuous-flow microPCR chips |
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58 | (4) |
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PCR inhibition and carryover contamination |
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62 | (3) |
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65 | (8) |
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73 | (2) |
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75 | (6) |
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5 High-Resolution Melt Profiling |
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81 | (33) |
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81 | (1) |
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Basic concepts of melt profiling |
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82 | (2) |
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HRMP and polymerase chain reaction |
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84 | (5) |
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89 | (1) |
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Determining a temperature window for melting |
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90 | (2) |
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Dyes and platforms for melt profiling |
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92 | (1) |
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Scanning PCR products for sequence variation |
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93 | (9) |
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Genotyping with high-resolution melt profiling |
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102 | (6) |
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Other applications of HRMP |
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108 | (1) |
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109 | (1) |
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110 | (4) |
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6 Massively Parallel Sequencing |
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114 | (21) |
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Massively parallel sequencing |
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114 | (1) |
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Commercially available massively parallel sequencers |
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115 | (7) |
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122 | (1) |
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Paired-end or mate-paired reads |
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123 | (1) |
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Target-enrichment strategies for MPS |
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124 | (1) |
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125 | (6) |
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131 | (1) |
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132 | (3) |
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7 Aptamers for Analysis: Nucleic Acids Ligands in the Post-Genomic Era |
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135 | (40) |
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135 | (1) |
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136 | (11) |
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147 | (10) |
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157 | (3) |
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Conclusions, outlooks and perspectives |
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160 | (1) |
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161 | (14) |
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8 Use of Nanotechnology for Enhancing of Cancer Biomarker Discovery and Analysis: A Molecular Approach |
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175 | (18) |
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175 | (1) |
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Proteomics and nanotechnology |
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176 | (1) |
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Nanoscale multicomponent separation |
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176 | (2) |
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Nanoscale protein detection strategies |
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178 | (4) |
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Surface-enhanced Raman scattering (SERS) |
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182 | (6) |
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188 | (5) |
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193 | (26) |
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Mikhail Soloviev Introduction |
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194 | (2) |
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196 | (9) |
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Chip-based mass spectrometry |
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203 | |
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Surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) chip instruments |
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205 | (2) |
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207 | (2) |
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209 | (2) |
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211 | (8) |
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10 Antibody Microarrays in Proteome Profiling |
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219 | (25) |
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Mohamed Saiel Saeed Alhamdani |
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Jorg D. Hoheisel Introduction |
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220 | (6) |
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Antibody array applications |
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226 | (3) |
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229 | (1) |
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229 | (1) |
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229 | (15) |
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11 Biomarker Detection and Molecular Profiling by Multiplex Microbead Suspension Array Based Immunoproteomics |
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244 | (27) |
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244 | (1) |
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Principles of microbead-based multiplexing |
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245 | (2) |
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Experimental aspects of the multiplex microbead assay |
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247 | (3) |
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Multiplex microbead assay design and comparison with other methods |
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250 | (2) |
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Applications of the multiplex microbead assay system for biomedical research and clinical studies |
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252 | (3) |
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Selected investigational fields for multiplex analysis and examples of applications |
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255 | (1) |
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Challenges and current limitations |
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255 | (4) |
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Summary and future directions |
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259 | (2) |
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261 | (1) |
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261 | (10) |
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12 Mass Spectrometry in Metabolomics |
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271 | (28) |
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271 | (1) |
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Sample collection and preparation |
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272 | (2) |
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274 | (5) |
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279 | (4) |
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283 | (11) |
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294 | (1) |
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294 | (1) |
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295 | (4) |
| Index |
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299 | |
Lisainfo e-raamatute kohta